Imagine that an RNA polymerase is transcribing a segment of DNA that contains the sequence:

5'- AGTCTAGGCACTGA -3'

3'- TCAGATCCGTGACT -5'

If the polymerase is transcribing this segment of DNA from left to right, which strand is the template?
[ Select ]
Top
Bottom

What will be the sequence of that RNA? 5'-
[ Select ]
TCAGATCCGTGACT
AGTCTAGGCACTGA
AGUCUAGGCACUGA
UCAGUGCCUAGACU
-3'

Question

Imagine that an RNA polymerase is transcribing a segment of DNA that contains the sequence:

5'- AGTCTAGGCACTGA -3'

3'- TCAGATCCGTGACT -5'

If the polymerase is transcribing this segment of DNA from left to right, which strand is the template?  
[ Select ]
Top
Bottom

What will be the sequence of that RNA?     5'-  
[ Select ]
TCAGATCCGTGACT
AGTCTAGGCACTGA
AGUCUAGGCACUGA
UCAGUGCCUAGACU
 -3'

BlackTom AI · Accepted Answer

We start by restating the question and the two dropdown choices involved.
Question: Imagine that an RNA polymerase is transcribing a segment of DNA that contains the sequence:

5'- AGTCTAGGCACTGA -3'
3'- TCAGATCCGTGACT -5'
If the polymerase is transcribing this segment of DNA from left to right, which strand is the template?  [ Select ] Top Bottom
What will be the sequence of that RNA?     5'-  [ Select ]
TCAGATCCGTGACT
AGTCTAGGCACTGA
AGUCUAGGCACUGA
UCAGUGCCUAGACU
 -3'

Option analysis for the first dropdown (template strand):
- Bottom: This is the correct choice. The polymerase reads the template strand in the 3'→5' direction and synthesizes RNA in the 5'→3' direction. If transcription proceeds left to right along the given sequence, the strand read by the polymerase is the bottom strand, which is oriented 3' to 5' in the segment shown, making it the template strand.
- Top: This would be incorrect. The top strand is oriented 5' to 3' in the segment provided. If transcription were to use the top strand as the template, the polymerase would have to read in the 3'→5' direction along that strand, which conflicts with the stated left-to-right transcription. Therefore, top cannot be the template strand in this setup.

Option analysis for the second dropdown (RNA sequence):
- AGUCUAGGCACUGA: This is the correct RNA sequence. When transcription uses the bottom strand as template, the resulting RNA sequence corresponds to the coding (top) strand with thymine replaced by uracil. The top DNA sequence is 5'-AGTCTAGGCACTGA-3'; replacing T with U gives 5'-AGUCUAGG CACUGA-3'. Note the spacing here reflects the exact nucleotide order: AGUCUAGGCACUGA.
- TCAGATCCGTGACT: This is a DNA sequence (contains thymine) and matches neither the RNA product nor the complementary relationship to the template in this scenario. It would not appear as the RNA transcript.
- AGTCTAGGCACTGA: This is the DNA coding (non-template) sequence with T instead of U, not the RNA transcript. RNA would have uracil in place of thymine, so this cannot be the RNA product.
- UCAGUGCCUAGACU: This RNA sequence does not match the expected complementary pairing to the bottom template or the top coding strand in the given orientation, so it represents a mispaired or misoriented transcript.

Putting it together, the two correct selections are Bottom for the template strand and AGUCUAGGCACUGA for the RNA sequence, consistent with transcription from the bottom template and RNA synthesis in the 5'→3' direction using the top strand as the coding reference.

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