Choose the RNA transcript that complements the DNA sequence 5'ATT-GTA-CTG-CAA3'

Question

BlackTom AI · Accepted Answer

RNA uses uracil (U) instead of thymine (T), and complementary base-pairing is A–U and T–A (for RNA–DNA), C–G and G–C. Given the DNA strand 5' ATT-GTA-CTG-CAA 3', the RNA strand that base-pairs with it must be antiparallel and complementary.

Write the DNA (given) left to right: 5' A T T - G T A - C T G - C A A 3'.
Find the complementary bases (pairing each DNA base with the RNA base that pairs to it):
- A (DNA) pairs with U (RNA)
- T (DNA) pairs with A (RNA)
- T → A
- G → C
- T → A
- A → U
- C → G
- T → A
- G → C
- C → G
- A → U
- A → U

This complementary sequence as written opposite the given DNA is 3' U A A - C A U - G A C - G U U 5' (note antiparallel orientation). To present the RNA transcript in the standard 5' → 3' direction, reverse that sequence to get 5' U U G - C A G - U A C - A A U 3'.

Compare to the choices: option (a) is exactly 5' UUG-CAG-UAC-AAU 3', matching the derived RNA transcript.

Why the other choices are incorrect:
- (b) and (d) contain T (thymine), which is a DNA base, not RNA, so they cannot be RNA transcripts.
- (c) 5' UAA-CAU-GAC-GUU 3' has the wrong order and several base mismatches (for example the first three bases are UAA but the correct first three are UUG), so it does not correctly complement the given DNA strand.

Therefore the correct RNA transcript is option a: 5' UUG-CAG-UAC-AAU 3', which matches the provided answer.

Choose the RNA transcript that complements the DNA sequence 5'ATT-GTA-CTG-CAA3'单项选择题

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