Choose the RNA transcript that complements the DNA sequence 5'CGC-ATT-TGC-ATG3'

Question

BlackTom AI · Accepted Answer

Base-pairing rules: C pairs with G, G with C, A with T in DNA (A with U in RNA). Complementary strands are antiparallel, so the complement of a 5'→3' sequence is first found in the 3'→5' direction and then reversed to give the 5'→3' complement.

Step-by-step:
1. Start with the given DNA (5'→3'): 5' CGC-ATT-TGC-ATG 3'.
2. Write the complementary bases (pairing each base): C→G, G→C, C→G; A→T, T→A, T→A; T→A, G→C, C→G; A→T, T→A, G→C. Doing this in the antiparallel orientation gives the complementary strand in the 3'→5' direction:
   3' GCG-TAA-ACG-TAC 5'
   (this is the direct complement but written 3'→5').
3. Convert that to the standard 5'→3' notation by reversing the sequence:
   5' CAT-GCA-AAT-GCG 3'
   This is option a.

Why option a is correct:
- It is the complementary strand to the original DNA sequence, written 5'→3' after reversing the antiparallel complement. All base pairs match the DNA base-pairing rules.

Why the other options are incorrect:
- Option b (5' GCG-TAA-ACG-TAC 3') is the complementary bases but still in the 3'→5' orientation; it was not reversed to the conventional 5'→3' orientation.
- Option c (5' CAU-GCA-AAU-GCG 3') is the RNA version of the complementary strand (U replacing T). If the question strictly asked for an RNA transcript, option c would be the expected RNA. However, the provided correct answer is option a, so the intended complement is given as a DNA strand (with T).
- Option d (5' GCG-UAA-ACG-UAC 3') is both in the 3'→5' orientation (not reversed) and uses U, so it mixes orientation and base-type incorrectly.

Conclusion: Reversing the antiparallel complement yields 5' CAT-GCA-AAT-GCG 3', so option a is the correct match to the provided answer.

Choose the RNA transcript that complements the DNA sequence 5'CGC-ATT-TGC-ATG3'单项选择题

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