A gene's coding (or non-template) strand is as follows: 5'-ATGTAGCAG. Which of the following would be the RNA product after transcription?

Question

BlackTom AI · Accepted Answer

Coding (non-template) DNA has the same sequence as the mRNA transcript except that thymine (T) in DNA is replaced by uracil (U) in RNA, and both are read 5' → 3'.

1) Relevant knowledge points
- Transcription uses the template (antisense) DNA strand (3' → 5') to synthesize RNA 5' → 3'.
- The coding (non-template, sense) strand is complementary to the template strand and therefore has the same sequence as the produced mRNA, except T in DNA → U in RNA.
- Pay attention to strand orientation (5' and 3').

2) Step-by-step reasoning
- Given coding strand (5' → 3'): ATGTAGCAG
- Replace each T with U to convert DNA coding to RNA sequence, preserving order and orientation.

3) Intermediate steps / simple conversion
- DNA coding: 5'- A T G T A G C A G -3'
- Change T → U: 5'- A U G U A G C A G -3'
- Thus mRNA = 5'-AUGUAGCAG

4) Why this is correct
- The mRNA is identical to the coding strand sequence because transcription produces an RNA strand complementary to the template (which is complementary to the coding strand). Replacing thymine with uracil yields the RNA sequence. The resulting 5'-AUGUAGCAG maintains correct base identity and 5'→3' orientation.

5) Why the other choices are incorrect
- 5'-CUGCUACAU: This is an RNA sequence but does not match the coding strand with T→U substitution; it appears to be a different (reverse/complement) sequence.
- 3'-CTGCTACAT: This is a DNA sequence written in 3' orientation and corresponds to the coding strand’s complement in 5'→3' orientation, not the RNA product.
- 3'-TACATCGTC: This exactly matches the template (antisense) DNA strand written 3'→5', not the RNA transcript.

6) Conclusion
The correct RNA product is 5'-AUGUAGCAG, which matches the provided answer.

A gene's coding (or non-template) strand is as follows: 5'-ATGTAGCAG. Which of the following would be the RNA product after transcription?单项选择题

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