Q1. What is the fractionation range (MW) for proteins when using Sephadex G100?单项选择题
A
a. 4,000 - 150,000
B
b. 10,000 - 10,000
C
c. 100 - 1,000
D
d. 0 - 150,000
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he gel filtration chromatogram shown above is from a mixture of proteins. Choose below the appropriate sizes for the peaks shown. The molecular mass is in kD.
The gel filtration chromatogram shown above is from a mixture of proteins. Choose below the appropriate sizes for the peaks shown. The molecular mass is in kD.
After finishing the purification of a DNA polymerase, researchers were surprised to find by gel filtration two peaks (70kD and 30kD) instead of one. An SDS-PAGE gel of the gel filtration fractions containing the peaks showed that indeed there were two proteins; one was identified as the DNA polymerase (30kD) and the other as a recombinase (40kD). A new purification scheme was devised that isolated the two proteins from each other as shown by gel filtration. The researchers hypothesized the proteins form a complex. The two purified proteins were mixed in a 1:1 ratio and gel filtration was used again. What would be your prediction of the gel filtration chromatography if the hypothesis were incorrect?
in the gel fltration chromatogram shown above, what kind of data would be needed to assess the correct mass of each of the peaks?
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