in the gel fltration chromatogram shown above, what kind of data would be needed to assess the correct mass of each of the peaks?

Question

BlackTom AI · Accepted Answer

To approach this question, consider what information gel filtration (size-exclusion chromatography) provides and what is required to assign a molecular weight to each peak.
Option 1: 'separate the proteins in the peaks in an SDS PAGE' — While SDS-PAGE can separate proteins by size and help identify components, using SDS-PAGE after the gel filtration run is not directly giving the masses of the peaks in the context of the chromatogram alone. It provides approximate sizes of isolated bands, but it does not link those sizes to the specific elution times without additional calibration steps. This step is often used for verification, not as the primary data needed to assign masses from the chromatogram itself.
Option 2: 'collect data on the mass of known proteins to compare to the experimental sample' — This aligns with standard practice: you run a set of protein standards with known molecular weights through the same gel filtration column to generate a calibration curve (elution volume or retention time vs. logMW). Then you compare the retention times of your sample peaks to the calibration curve to estimate their masses. This provides the necessary reference data to assign approximate masses to each peak.
Option 3: 'separate the proteins in the peaks in a different column' — Using a different column won’t directly yield the masses for the peaks observed in the original gel filtration run. Calibration with standards on the same column is essential; switching columns could introduce different void volumes and pore sizes, leading to incorrect mass estimates. This would not provide the required data to assess peak masses accurately.
Option 4: 'Perform an SDS-PAGE and repeat the experiment' — Repeating with SDS-PAGE again gives size information for individual bands, but it does not establish a direct, quantitative relationship between the chromatographic peak retention and molecular weight on the same calibration basis as a standard curve from the same column. It’s more of a separate technique, not the primary data set needed for assigning masses to the peaks observed in the gel filtration trace.
Putting these together, the core data needed to assign correct masses to the peaks is a set of known-mass standards run on the same column to build a calibration reference, which is described in Option 2.

in the gel fltration chromatogram shown above, what kind of data would be needed to assess the correct mass of each of the peaks? 单项选择题

类似问题

he gel filtration chromatogram shown above is from a mixture of proteins. Choose below the appropriate sizes for the peaks shown. The molecular mass is in kD.

The gel filtration chromatogram shown above is from a mixture of proteins. Choose below the appropriate sizes for the peaks shown. The molecular mass is in kD.

Q1. What is the fractionation range (MW) for proteins when using Sephadex G100?

Q7. Which of the two proteins do you expect to elute first from the Sephadex G100 column?

Q1. What is the fractionation range (MW) for proteins when using Sephadex G100?

The history of the Nation of Islam (NOI) reveals that they seek recruits from prison.

更多留学生实用工具

智能学习助手

风格化写作助手

论文查重助手

文献引用助手

课堂转译助手

课堂笔记助手

Quiz搜索助手

学校历年真题

智能刷题助手

智能匹配练习

希望你的学习变得更简单