In Phage display (select the correct answer):

Question

BlackTom AI · Accepted Answer

Question restatement: In Phage display (select the correct answer):

Option a: 'The antibodies produced by different hybridomas are pooled and selected by fishing them with an antigen attached to a magnetic bead.' This describes a hybridoma-based approach with bead-based selection, not phage display. Phage display uses a library of phage particles displaying peptide or antibody fragments, and selection occurs through binding to the target, not pooling hybridoma-derived antibodies. Therefore this option mischaracterizes the core mechanism of phage display.

Option b: 'The infection of a bacteria with phages containing the right antigen makes the bacteria produces the desired antibody.' In phage display, the purpose is not to infect bacteria to produce antibodies. Instead, phage particles display the antibody fragment on their surface, and the selection is based on affinity to the antigen. This statement conflates phage display with a different, impractical notion of producing antibodies by infection, making it incorrect.

Option c: 'The selection of an epitope-selective scFv among thousands of other non-selective variants (mutants) takes place in a 1.5 mL Eppendorf tube. That makes phage display one of the most efficient methods of screening/selection' This option correctly captures the essence of phage display: a large library of phage-displayed scFvs is subjected to binding to an antigen (panning), often in small-volume tubes, allowing rapid, efficient selection of specific binders from vast diversity. The reference to thousands of variants and the physical setup (small tube, efficient screening) aligns with standard phage display workflows.

Option d: 'The scFv molecule is an immortalised antibody that can resist the panning conditions without denaturing' This is inaccurate. scFv stands for single-chain variable fragment, not an immortalised antibody, and while stability is a consideration, there is no claim that scFvs are inherently immortal or completely resistant to all panning conditions. This statement overstates stability and misdefines the nature of scFv. It misleads about what scFv means and how it behaves under selection conditions.

In summary, the reasoning for each option shows that a and b describe concepts not aligned with phage display, d mischaracterizes what an scFv is and its stability, while c accurately reflects the phage display selection process and its efficiency in isolating specific binders from a large library.

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