Figure 3.3 in SKD 3rd Edition shows the effect of initial substrate concentration on the initial reaction rate (v) for a constant total enzyme concentration ([E0]). Now consider a plot of initial reaction rate (v) vs. total enzyme concentration ([E0]) for constant initial substrate concentration ([S0]). Which of the following describes the shape that would be obtained for such a plot?单项选择题

A

Horizontal line; [E0] has no effect on v.

B

Straight line with positive slope beginning at a value of v = 0 for [E0] = 0; v increases linearly with [E0].

C

For lower [E0] values, the curve will be a straight line with positive slope beginning at a value of v = 0 for [E0] = 0. However, at higher [E0] values the reaction rate (v) will increase more slowly with increasing [E0] and eventually approach a maximum value plateau.

D

None of the above.

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类似问题

Which of the following statements about V0, Vmax, [S] and Km values is not true?

The Vmax of an enzyme is:

Is the following statement true or false?'There is an inverse relationship between the Km and an enzyme’s affinity for its substrate'.

Question textFollow the instructions in your laboratory manual (page 24, Questions 1-4) to complete the following protocol table for this study on the effect of substrate concentration on enzyme activity. Give all your answers as numbers only. [table] Assay # | Final concentration of Ethanol(mM) | Volume of 0.6 M ethanol stock required(μL) | Volume of 15 mM NAD+ stock required to give final concentration of 0.5 mM(μL) | Volume of assay buffer(μL) | Water to provide a total volume of 290 μL(give your answer in μL) | Volume of ADH enzyme required(μL)(add last) | Total volume(μL) 1 | 0 | Answer 1 Question 14 | Answer 2 Question 14 | 200 | Answer 3 Question 14 | 10 | 300 2 | 5 | Answer 4 Question 14 | Answer 5 Question 14 | 200 | Answer 6 Question 14 | 10 | 300 3 | 10 | 回答第7题第14题 | 回答第 8 题第 14 题 | 200 | 答案 9 问题 14 | 10 | 300 4 | 14 | 回答第10题第14题 | 答案 11 问题 14 | 200 | 答案 12 问题 14 | 10 | 300 5 | 20 | 答案 13 问题 14 | 答案 14 问题 14 | 200 | 答案 15 问题 14 | 10 | 300 6 | 30 | 答案 16 问题 14 | 答案 17 问题 14 | 200 | 答案 18 问题 14 | 10 | 300 7 | 50 | 答案 19 问题 14 | 回答第 20 题第 14 题 | 200 | 答案 21 问题 14 | 10 | 300 8 | 100 | 答案 22 问题 14 | 答案 23 问题 14 | 200 | 答案 24 问题 14 | 10 | 300 [/table]

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