The Vmax of an enzyme is:单项选择题

题目图片
A

a. the maximal rate of reaction

B

b. half the maximal rate of reaction

C

c. the concentration of substrate that gives the maximal rate of reaction

D

d. the concentration of substrate that gives half the maximal rate of reaction

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类似问题

Which of the following statements about V0, Vmax, [S] and Km values is not true?

Is the following statement true or false?'There is an inverse relationship between the Km and an enzyme’s affinity for its substrate'.

Question textFollow the instructions in your laboratory manual (page 24, Questions 1-4) to complete the following protocol table for this study on the effect of substrate concentration on enzyme activity. Give all your answers as numbers only. [table] Assay # | Final concentration of Ethanol(mM) | Volume of 0.6 M ethanol stock required(μL) | Volume of 15 mM NAD+ stock required to give final concentration of 0.5 mM(μL) | Volume of assay buffer(μL) | Water to provide a total volume of 290 μL(give your answer in μL) | Volume of ADH enzyme required(μL)(add last) | Total volume(μL) 1 | 0 | Answer 1 Question 14 | Answer 2 Question 14 | 200 | Answer 3 Question 14 | 10 | 300 2 | 5 | Answer 4 Question 14 | Answer 5 Question 14 | 200 | Answer 6 Question 14 | 10 | 300 3 | 10 | 回答第7题第14题 | 回答第 8 题第 14 题 | 200 | 答案 9 问题 14 | 10 | 300 4 | 14 | 回答第10题第14题 | 答案 11 问题 14 | 200 | 答案 12 问题 14 | 10 | 300 5 | 20 | 答案 13 问题 14 | 答案 14 问题 14 | 200 | 答案 15 问题 14 | 10 | 300 6 | 30 | 答案 16 问题 14 | 答案 17 问题 14 | 200 | 答案 18 问题 14 | 10 | 300 7 | 50 | 答案 19 问题 14 | 回答第 20 题第 14 题 | 200 | 答案 21 问题 14 | 10 | 300 8 | 100 | 答案 22 问题 14 | 答案 23 问题 14 | 200 | 答案 24 问题 14 | 10 | 300 [/table]

Question textFollow the instructions in your laboratory manual (page 24, Questions 1-4) to complete the following protocol table for this study on the effect of substrate concentration on enzyme activity. Give all your answers as numbers only. [table] Assay # | Final concentration of Ethanol(mM) | Volume of 0.6 M ethanol stock required(μL) | Volume of 15 mM NAD+ stock required to give final concentration of 0.5 mM(μL) | Volume of assay buffer(μL) | Water to provide a total volume of 290 μL(give your answer in μL) | Volume of ADH enzyme required(μL)(add last) | Total volume(μL) 1 | 0 | Answer 1 Question 14 | Answer 2 Question 14 | 200 | Answer 3 Question 14 | 10 | 300 2 | 5 | Answer 4 Question 14 | Answer 5 Question 14 | 200 | Answer 6 Question 14 | 10 | 300 3 | 10 | Answer 7 Question 14 | Answer 8 Question 14 | 200 | Answer 9 Question 14 | 10 | 300 4 | 14 | Answer 10 Question 14 | Answer 11 Question 14 | 200 | Answer 12 Question 14 | 10 | 300 5 | 20 | Answer 13 Question 14 | Answer 14 Question 14 | 200 | Answer 15 Question 14 | 10 | 300 6 | 30 | Answer 16 Question 14 | Answer 17 Question 14 | 200 | Answer 18 Question 14 | 10 | 300 7 | 50 | Answer 19 Question 14 | Answer 20 Question 14 | 200 | Answer 21 Question 14 | 10 | 300 8 | 100 | Answer 22 Question 14 | Answer 23 Question 14 | 200 | Answer 24 Question 14 | 10 | 300 [/table]Check Question 14

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