Gottschalk (and Kelley) note that the Protein A capture step is generally robust, but some [of its] variables may still be influenced by product or feed-stream variations. Which option best lists those variables?

Question

BlackTom AI · Accepted Answer

To tackle the question, I’ll evaluate what Gottschalk and Kelley identify as variables in the Protein A capture step that could be affected by feed- or product-stream variations.
Option 1: 'Culture duration, feeding strategy, and cell density' — these factors are more typically associated with upstream processing and cell culture performance, not the Protein A capture chemistry itself. They influence what is fed into the downstream unit, but they’re not intrinsic variables of the Protein A capture step.
Option 2: 'Column bed height, resin lifetime, and membrane flux' — column bed height relates to column geometry, resin lifetime to how long the resin remains active, and membrane flux to filtration steps; while relevant to chromatography and overall process performance, they are not the classic variable triad described for Protein A capture susceptibility to feed/product variation. These terms mix chromatography and filtration concepts, making this option less coherent as a single capture-step variable set.
Option 3: 'Dynamic binding capacity, column wash solution composition, and elution conditions' — this set directly targets the Protein A capture step: dynamic binding capacity (how much protein can be bound under current conditions), wash solution composition (affects removal of impurities while preserving bound product), and elution conditions (pH, salt, and other factors that release the product from Protein A). These are the core levers that could shift with product or feed variations and are commonly discussed in capture-process robustness analyses.
Option 4: 'Clarification time, depth filter area, and virus filter integrity' — these terms pertain to clarification and downstream filtration/sterilization stages, not the Protein A capture step itself. They do not describe variables inherent to the Protein A resin capture under varying feed/product conditions.
From this breakdown, only Option 3 aligns with the expected variables for the Protein A capture step being influenced by upstream variations, while the others concern either upstream culture parameters or downstream unit operations rather than the capture chemistry itself. Thus, Option 3 correctly identifies the key variables that would be sensitive to product or feed-stream changes during the Protein A capture stage.

Gottschalk (and Kelley) note that the Protein A capture step is generally robust, but some [of its] variables may still be influenced by product or feed-stream variations. Which option best lists those variables?单项选择题

类似问题

Protein A column regeneration is typically carried out with:

Which statement is NOT true about Protein A as an industry-standard capture resin?

Why are alternatives to Protein A capture being actively pursued?

In Fig. 5.2, the elution peak is narrow and tall. What does this most strongly indicate?

Protein A column regeneration is typically carried out with:

Nonspecific binding is rarely minimized in practice primarily because:

§5.3 states the simplest general approach to reduce aggregation/precipitation during Protein A elution is often:

Which one would be the best choice to use for an affinity chromatography to purify a DNA polymerase?

更多留学生实用工具

智能学习助手

风格化写作助手

论文查重助手

文献引用助手

课堂转译助手

课堂笔记助手

Quiz搜索助手

学校历年真题

智能刷题助手

智能匹配练习

希望你的学习变得更简单