A template strand of DNA reads 3' C T G A 5'. The RNA transcript of this strand would read

Question

BlackTom AI · Accepted Answer

To determine the RNA transcript, we must remember that RNA polymerase reads the DNA template strand in the 3' to 5' direction and synthesizes RNA in the 5' to 3' direction. The RNA is complementary to the template and replaces thymine (T) with uracil (U).

Option 1: 5' A G U C 3'. This would imply a sequence pairing with T opposite A, but the first nucleotide should pair with the 3' end of the template, which is C; C pairs with G in RNA, not with A. So this is not correct.

Option 2: 5' G A C U 3'. This sequence is complementary to the template 3' C T G A 5' when read in the proper orientation: C pairs with G, T with A, G with C, A with U. Therefore, this matches the expected RNA transcript.

Option 3: 5' C U G A 3'. This sequence would pair with the template in a manner inconsistent with the required 5' to 3' synthesis direction and would not be the correct complementary sequence to 3' C T G A 5'. Thus, incorrect.

Option 4: 5' U C A G 3'. This sequence does not align with the base-pairing rules for the given template; U would pair with A, not with the template end provided, making this option incorrect.

In summary, considering directionality and base-pairing rules (C→G, T→A, G→C, A→U) and the 5' to 3' synthesis of RNA, the correct transcript is 5' G A C U 3'.

A template strand of DNA reads 3' C T G A 5'. The RNA transcript of this strand would read单项选择题

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