In the process of creating animal-free monoclonal antibodies using phage display, which of the following steps is correctly described?

Question

BlackTom AI · Accepted Answer

Consider the overall workflow of creating animal-free monoclonal antibodies via phage display. 
Option a) Phages displaying antibody fragments are propogated using E. coli. This aligns with the standard method: M13 phage particles displaying antibody fragments are produced and amplified inside E. coli, enabling replication and propagation of the phage-displayed library. The use of E. coli is essential for phage replication and library propagation.
Option b) All phages are immediately discarded, and only unbound fragments are analyzed. In reality, after binding (panning), bound phages are eluted and then amplified in E. coli for further rounds; simply discarding all phages and analyzing unbound fragments would discard the very pool that contains potential binders, which is incorrect.
Option c) Washing is done to enhance binding between phages and nonspecific targets. On the contrary, washes are intended to remove weakly bound or non-specific phages, reducing background and enriching for phages with genuine affinity. The goal is to improve specificity, not to promote nonspecific binding.
Option d) The weakest binding phages are typically selected as the final antibodies. This is opposite to the objective of phage display panning, which favors strong, specific binders through iterative rounds; weak binders are usually discarded or fail to enrich over successive rounds.

In the process of creating animal-free monoclonal antibodies using phage display, which of the following steps is correctly described?单项选择题

类似问题

Phage display technology is primarily used to:

Phage display technology is primarily used to:噬菌体展示技术主要被用于：

Which of the following allows for animal-free monoclonal antibody creation?

In Phage display (select the correct answer):

Which of the following trees corresponds to a potential parse of the ambiguous sentence below, with correct syntactic categories? Some diagnostics are provided.

Which of the following sentences contain two non-finite verbs? Select all that apply.

Analyze the following sentence: The sentence contains [ Select ] 8 6 5 7 3 4 functional words, [ Select ] 2 3 1 4 5 count nouns, [ Select ] 4 5 2 1 3 mass nouns, and [ Select ] 1 5 2 3 4 adjectives.

更多留学生实用工具

智能学习助手

风格化写作助手

论文查重助手

文献引用助手

课堂转译助手

课堂笔记助手

Quiz搜索助手

学校历年真题

智能刷题助手

智能匹配练习

希望你的学习变得更简单