Which of the following statements about V0, Vmax, [S] and Km values is not true?单项选择题

题目图片
A

a. An enzyme with a Km value much lower than the concentration of its substrate in the cell (Km << [S]) would be well suited to a ‘housekeeping’ role that needs to be maintained all the time.

B

b. An enzyme with a high Vmax necessarily has a high Km.

C

c. An enzyme with a Km value much higher than the concentration of its substrate in the cell (Km >> [S]) would be well suited to a role that needs to respond to variations in [S].

D

d. Many enzymes have a Km fairly close to the concentration of their substrate in the cell, so they usually work at a significant fraction of their Vmax but can respond to changes in [S] if required.

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类似问题

The Vmax of an enzyme is:

Is the following statement true or false?'There is an inverse relationship between the Km and an enzyme’s affinity for its substrate'.

Question textFollow the instructions in your laboratory manual (page 24, Questions 1-4) to complete the following protocol table for this study on the effect of substrate concentration on enzyme activity. Give all your answers as numbers only. [table] Assay # | Final concentration of Ethanol(mM) | Volume of 0.6 M ethanol stock required(μL) | Volume of 15 mM NAD+ stock required to give final concentration of 0.5 mM(μL) | Volume of assay buffer(μL) | Water to provide a total volume of 290 μL(give your answer in μL) | Volume of ADH enzyme required(μL)(add last) | Total volume(μL) 1 | 0 | Answer 1 Question 14 | Answer 2 Question 14 | 200 | Answer 3 Question 14 | 10 | 300 2 | 5 | Answer 4 Question 14 | Answer 5 Question 14 | 200 | Answer 6 Question 14 | 10 | 300 3 | 10 | 回答第7题第14题 | 回答第 8 题第 14 题 | 200 | 答案 9 问题 14 | 10 | 300 4 | 14 | 回答第10题第14题 | 答案 11 问题 14 | 200 | 答案 12 问题 14 | 10 | 300 5 | 20 | 答案 13 问题 14 | 答案 14 问题 14 | 200 | 答案 15 问题 14 | 10 | 300 6 | 30 | 答案 16 问题 14 | 答案 17 问题 14 | 200 | 答案 18 问题 14 | 10 | 300 7 | 50 | 答案 19 问题 14 | 回答第 20 题第 14 题 | 200 | 答案 21 问题 14 | 10 | 300 8 | 100 | 答案 22 问题 14 | 答案 23 问题 14 | 200 | 答案 24 问题 14 | 10 | 300 [/table]

Question textFollow the instructions in your laboratory manual (page 24, Questions 1-4) to complete the following protocol table for this study on the effect of substrate concentration on enzyme activity. Give all your answers as numbers only. [table] Assay # | Final concentration of Ethanol(mM) | Volume of 0.6 M ethanol stock required(μL) | Volume of 15 mM NAD+ stock required to give final concentration of 0.5 mM(μL) | Volume of assay buffer(μL) | Water to provide a total volume of 290 μL(give your answer in μL) | Volume of ADH enzyme required(μL)(add last) | Total volume(μL) 1 | 0 | Answer 1 Question 14 | Answer 2 Question 14 | 200 | Answer 3 Question 14 | 10 | 300 2 | 5 | Answer 4 Question 14 | Answer 5 Question 14 | 200 | Answer 6 Question 14 | 10 | 300 3 | 10 | Answer 7 Question 14 | Answer 8 Question 14 | 200 | Answer 9 Question 14 | 10 | 300 4 | 14 | Answer 10 Question 14 | Answer 11 Question 14 | 200 | Answer 12 Question 14 | 10 | 300 5 | 20 | Answer 13 Question 14 | Answer 14 Question 14 | 200 | Answer 15 Question 14 | 10 | 300 6 | 30 | Answer 16 Question 14 | Answer 17 Question 14 | 200 | Answer 18 Question 14 | 10 | 300 7 | 50 | Answer 19 Question 14 | Answer 20 Question 14 | 200 | Answer 21 Question 14 | 10 | 300 8 | 100 | Answer 22 Question 14 | Answer 23 Question 14 | 200 | Answer 24 Question 14 | 10 | 300 [/table]Check Question 14

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