Question textFollow the instructions in your laboratory manual (page 24, Questions 1-4) to complete the following protocol table for this study on the effect of substrate concentration on enzyme activity. Give all your answers as numbers only. [table] Assay # | Final concentration of Ethanol(mM) | Volume of 0.6 M ethanol stock required(μL) | Volume of 15 mM NAD+ stock required to give final concentration of 0.5 mM(μL) | Volume of assay buffer(μL) | Water to provide a total volume of 290 μL(give your answer in μL) | Volume of ADH enzyme required(μL)(add last) | Total volume(μL) 1 | 0 | Answer 1 Question 14 | Answer 2 Question 14 | 200 | Answer 3 Question 14 | 10 | 300 2 | 5 | Answer 4 Question 14 | Answer 5 Question 14 | 200 | Answer 6 Question 14 | 10 | 300 3 | 10 | Answer 7 Question 14 | Answer 8 Question 14 | 200 | Answer 9 Question 14 | 10 | 300 4 | 14 | Answer 10 Question 14 | Answer 11 Question 14 | 200 | Answer 12 Question 14 | 10 | 300 5 | 20 | Answer 13 Question 14 | Answer 14 Question 14 | 200 | Answer 15 Question 14 | 10 | 300 6 | 30 | Answer 16 Question 14 | Answer 17 Question 14 | 200 | Answer 18 Question 14 | 10 | 300 7 | 50 | Answer 19 Question 14 | Answer 20 Question 14 | 200 | Answer 21 Question 14 | 10 | 300 8 | 100 | Answer 22 Question 14 | Answer 23 Question 14 | 200 | Answer 24 Question 14 | 10 | 300 [/table]Check Question 14Multiple fill-in-the-blank