What is true regarding the beta-galactosidase assay? Select all correct answers.多项选择题

A

One uses a spec to estimate the number of cells in the culture when doing this procedure.

B

Serratia marcescens is what we will be working with.

C

It assesses how many membranes a bacterium contains.

D

The solution turns blue to indicate the reaction is complete.

E

The solution turns yellow to indicate the reaction is copmlete.

F

Cells will be grown in minimal media with either glucose or lactose

G

It measures product formation of ONP over time

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This is an enzyme, lagunase. Lagunase converts NAD+ and ethanol into aldehyde and NADH. Lagunase needs two Zn2+ ions in order to function. Design an assay for lagunase. What will you measure? What will you add to the mixture to start the rxn?  What will your data give you?

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