You and your lab partner Marlin are running an activity assay for LDH. You determine the proper dilution for your sample and are collecting your replicates.  The first two activity assays are collected properly, however after collecting the 60s time point on the third assay, you tell your lab partner a joke involving a mollusk and a sea cucumber. Marlin is laughing so hard that he completely misses the 90s time point and resumes collecting at the 120s time point.  Do you and your lab partner need to run an extra activity assay to make up for the missing data point?单项选择题

A

Most likely not, since you have already determined the proper dilution, it is likely that the overall shape of the trendline will be curved as expected

B

Most likely not, since you have already determined the proper dilution, it is likely that the overall slope of the data points will remain unchanged

C

Most likely, without that data point, your slope calculation is likely to be significantly changed as a result of the missed data point

D

Most likely, since the lab manual requires the 90s time point, that time point is indispensable and your calculated activity concentration will be incorrect.

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