Which of the following are steps involved in a standard somatic mutation calling pipeline using a tumor-normal pair? (Select all that apply)多项选择题

A

Call germline variants using bcftools

B

Map normal and tumor sequencing reads to the reference genome

C

Subtract the tumor variants from the normal variants to find the reference sequence

D

Call variants using Mutect2

E

Install GATK (Mutect2), BWA-MEM, and SAMtools

F

Filter high-quality somatic variants

G

Retain only the variants that are common between the normal and tumor samples

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