In the original publication of the 4V42 ribosome structure (Yusupov et al., 2001), the researchers included an image of all three tRNAs as well as the mRNA (much like you did, though perhaps from a different angle). The authors say, regarding this image, that "the simultaneous reading of the two codons is accommodated by a kink in the mRNA backbone of about 45o between the A and P codons": They also say something that is more difficult to see in this image, so think back to what you yourself observed as well as you were creating your own image: "The A- and P-tRNA backbones are closest at the acceptor stems, which approach within 5 Angstroms of each other.... The CCA tails of the A- and P-tRNAs converge, as expected, at their 3' ends in the peptidyl transferase site of the [large] subunit." Meanwhile they note that the anticodons of the A- and P-tRNAs are approximately 10 Angstroms apart. Your own experience manipulating the 4V42 structure file plus this description above should make it clear why it is difficult to show translation in the ribosome as a series of flat 2D representations: it's a highly 3D process. As a review, if the 4V42 structure file (and corresponding experiment) had been able to contain tRNAs with amino acids attached, where would they be? Let's assume that only two codons have been read so far. Choose the best answer below. The amino acids would be attached to the tRNAs that are in the: 单项选择题

A
E site and P site; in the figure above they would be at the back and obscured from view
B
P site and A site; in the figure above they would be at the back and obscured from view
C
E site and P site; in the figure above they would be at the bottom in contact with the mRNA
D
P site and A site; in the figure above they would be at the bottom in contact with the mRNA
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Take this sequence and find the start site, the stop site for translation. Then write down the first 20 amino acids. (To do this scan down the DNA until you find the first ATG, then section the sequence into triplets until you find a stop codon). To translate this gene into a protein, use the above codon table. Use the 1 letter code for your answer now spaces. For example MALLS. A correct answer will have 20 uppercase letters in it
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