Which of the following is an antibody that is a suitable positive control in an ELISA test for the autoimmune disease Systemic Lupus Erythematosus (SLE)?

Question

BlackTom AI · Accepted Answer

Anti-dsDNA antibodies are a hallmark serologic marker for Systemic Lupus Erythematosus (SLE), so an anti-DNA primary antibody is the appropriate positive control for an ELISA targeting SLE autoantibodies.

1) Relevant knowledge points and concepts
- SLE is classically associated with autoantibodies against double-stranded DNA (anti-dsDNA) and Sm antigen; these are used diagnostically.
- In an indirect ELISA for autoimmune antibodies, the plate is coated with the specific self-antigen (e.g., DNA). Patient sera are added; any antigen-specific antibodies bind. A labeled secondary (anti-human Ig) detects bound patient antibodies.
- A positive control should be a known antibody that binds the same antigen used in the assay (here, DNA) to verify coating, binding, and detection steps work.

2) Step-by-step reasoning
- Step 1: Identify the disease-specific antigen: for SLE, DNA is a primary specific antigen.
- Step 2: Choose a control reagent that will definitely bind that antigen: a validated anti-DNA primary antibody (monoclonal or polyclonal) will bind coated DNA.
- Step 3: Run the ELISA with the anti-DNA reagent as the positive control. A measurable signal confirms antigen coating and detection chemistry are functioning.

3) Intermediate steps (logical progression)
- Coat plate with DNA → add anti-DNA primary antibody (positive control) → add labeled secondary antibody recognizing the primary → develop substrate → observe signal. Each step isolates functionality: antigen presentation, antibody binding, secondary detection, and signal generation.

4) Why the correct answer is correct
- Anti-DNA primary antibody specifically targets the SLE-relevant antigen and will produce a reliable positive signal in this ELISA format, validating the assay for detecting anti-DNA antibodies in patient samples.

5) Why other options are incorrect
- Anti-Rheumatoid Factor (RF): RF is associated with rheumatoid arthritis, not SLE, so it is not a disease-specific positive control for SLE ELISA.
- Anti-Proteolipid Protein (PLP): PLP antibodies are related to multiple sclerosis/myelin disorders, irrelevant to SLE.
- Anti-Acetylcholine Receptor (AChR): AChR antibodies are diagnostic for myasthenia gravis, not SLE.

6) Conclusion
Therefore, the correct positive control is an Anti-DNA primary antibody. This matches the provided answer: Anti-DNA primary antibody.

Which of the following is an antibody that is a suitable positive control in an ELISA test for the autoimmune disease Systemic Lupus Erythematosus (SLE)?单项选择题

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