Which of the following are valid methods for detecting doublets in single-cell RNA-seq experiments? (Select ALL that apply)多项选择题

Using hashtag oligonucleotide (HTO) antibody barcodes to label cells from different samples

Loading cells from two different species and identifying mixed transcriptomes

Reducing the cell loading concentration to lower doublet rate

Using genetic barcoding (demuxlet) to identify cells with mixed genotypes from different individuals

Increasing the number of PCR amplification cycles