A scientist who is studying the XYZ gene in a eukaryotic model cell inserts an miRNA that is complementary to a portion of the XYZ mRNA found in the cell. What result would you predict?

Question

A scientist who is studying the XYZ gene in a eukaryotic model cell inserts an miRNA that is complementary to a portion of the XYZ mRNA found in the cell. What result would you predict?

BlackTom AI · Accepted Answer

The scenario involves introducing an miRNA that is complementary to a portion of the XYZ mRNA in a eukaryotic cell. This setup targets post-transcriptional regulation.
Option 1: 'There will be an increase in transcription of the XYZ gene.' miRNA interacts with mRNA after transcription has occurred; it does not typically upregulate transcription. Transcriptional changes would require factors acting at the DNA level or chromatin state, not miRNA binding to mRNA.
Option 2: 'No change will result from the insertion of the miRNA' In most cases, an miRNA complementary to XYZ mRNA will affect mRNA stability or translation, leading to a measurable effect on protein levels. Therefore, saying there is no change ignores the canonical role of miRNAs in gene silencing.
Option 3: 'There will be a decrease in transcription of the XYZ gene.' Similar to Option 1, miRNAs act post-transcriptionally; they do not typically suppress transcription itself. A decrease in transcription would imply changes at the promoter or chromatin level, which is not the mechanism described here.
Option 4: 'There will be a decrease in the amount of XYZ protein made.' This aligns with the usual function of miRNAs: binding to target mRNA can block translation and/or promote mRNA degradation, reducing protein output.
Option 5: 'There will be an increase in the amount of XYZ protein made.' This is contrary to the inhibitory role of an miRNA complementary to XYZ mRNA; it would not be expected to enhance protein production.
In summary, the miRNA targeting XYZ mRNA is expected to lower protein production by interfering with mRNA stability or translation, making Option 4 the correct interpretation.

A scientist who is studying the XYZ gene in a eukaryotic model cell inserts an miRNA that is complementary to a portion of the XYZ mRNA found in the cell. What result would you predict?单项选择题

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