Question textUsing the plasmid map of pOTC-Δ provided above, calculate the sizes of the DNA fragments that you would expect to see on your agarose gel following a restriction digest using KpnI alone, NdeI and NotI, and SphI and NheI, and record your answers in the spaces provided in the table below.This question will be automatically graded so it is essential that you adhere to the following formatting of your answers: use numbers only (i.e. '50', not 'fifty') do not include any units (i.e. base pairs, bp) if there is more than one number to be entered, use a comma then a space (', ') to separate multiple numbers e.g. if your answer is 1566 and 2009, write it as 1566, 2009 do not add a full stop or any other punctuation after your answer [table] | KpnI alone | NdeI and NotI | SphI and NheI Position(s) where each enzyme will cut: (bp# positions) | KpnI Answer 1 Question 6 | NdeI Answer 2 Question 6 NotI Answer 3 Question 6 | SphI Answer 4 Question 6 NheI Answer 5 Question 6 Calculations of expected fragment lengths: (in length units of bp) | Answer 6 Question 6 | Answer 7 Question 6 | Answer 8 Question 6 [/table]多项填空题