Which of the following techniques gives information about the concentration of a protein?

Question

BlackTom AI · Accepted Answer

To begin, let's evaluate what each technique typically measures in the context of proteins.
Option a: Mass spectrometry is primarily used to determine molecular mass, identify proteins, and characterize fragments or modifications, not to quantify total protein concentration directly. It provides qualitative and some quantitative insights under specialized conditions, but that is not its main or most straightforward use for concentration measurement.
Option b: Circular dichroism observes how chiral molecules absorb circularly polarized light, which is mainly used to assess secondary structure content (alpha-helix, beta-sheet) and folding, rather than giving a direct measure of how much protein is present in a sample. It is not a concentration assay.
Option c: SDS-PAGE gel electrophoresis separates proteins mainly by molecular weight under denaturing conditions and is typically used to assess purity and approximate sizes. While you can estimate relative amounts by staining intensity, SDS-PAGE is not a direct, reliable method for determining absolute protein concentration without a standard curve, making it an indirect and less preferred choice for concentration quantification.
Option d: UV-Vis spectroscopy involves measuring absorbance of light, commonly at 280 nm for proteins due to aromatic amino acids like tryptophan and tyrosine. This method is widely used to determine protein concentration using Beer-Lambert Law with a proper extinction coefficient, making it the standard technique for concentration measurements.
In summary, the primary function of each technique aligns with structure, mass, or separation aspects, but UV-Vis spectroscopy uniquely provides a direct route to quantify protein concentration through absorbance measurements that relate to concentration via a known extinction coefficient.

Which of the following techniques gives information about the concentration of a protein?单项选择题

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