As cell-culture titers increase, which statement is NOT consistent with the passage?

Question

BlackTom AI · Accepted Answer

Dynamic binding capacity (DBC) is the amount of product a resin can bind under given conditions, while loading flow rate and column cycle time determine overall throughput. At higher cell-culture titers the bottleneck shifts from how much each binding site can hold (DBC) toward how quickly the capture step can be executed and how many cycles are required, so process choices favor maximizing productive cycles and flow rates over chasing marginal increases in DBC.

Step-by-step reasoning:
1. With rising titers, target mass of product per batch increases. To process that mass efficiently you either increase column capacity per cycle or reduce cycle time. Pushing the Protein A step toward maximum loading capacity reduces the number of cycles needed and is therefore desirable (option 1 is consistent).
2. Shorter cycle times are achieved by increasing loading flow rate; when titers are high, time-per-cycle (throughput) becomes the dominant constraint, so maximizing loading flow rate becomes more important than squeezing out more DBC (option 2 is consistent).
3. Therefore resin selection emphasis shifts away from DBC alone and toward resins that support faster loading, higher flow, and stable performance under shorter cycles. A statement that resin choice is based more on DBC contradicts this shift and is NOT consistent with the passage (option 3 is the incorrect/NOT consistent statement).
4. The passage’s logic implies resin choice is less about static capacity metrics and more about operational performance (cycle time and loading behavior), so saying resin choice is based less on how fast the column can be loaded would contradict the throughput-driven focus—however, in context the passage emphasizes deprioritizing DBC relative to loading speed, making option 4 consistent with the overall point that operational parameters (including how fast you can load) govern resin choice.

Why the correct answer is correct: Option 3 directly contradicts the passage’s central claim that, as titers rise, DBC becomes a less dominant selection criterion. Hence “Resin choice is based more on DBC” is NOT consistent with the passage. This matches the provided answer.

As cell-culture titers increase, which statement is NOT consistent with the passage?单项选择题

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As cell-culture titers increase, which statement is NOT consistent with the passage?单项选择题

类似问题

A UF/DF step before Protein A. Which statement is NOT correct?

In an alternative placement described in Chapter 11.4, precipitation is introduced to replace polishing steps, but it has challenges. Which statement is NOT consistent with Chapter 11.4?

Adding precipitating agent after the cells have been removed has added value because it can:

According to Chapter 11, precipitation strategy is best described as:

For maximum removal of nonspecifically bound material, the intermediate wash pH should be:

A UF/DF step before Protein A. Which statement is NOT correct?

A UF/DF step before Protein A is most justified when:

You run a gel after a purification experiment. This gel is shown above: The lane that most likely represents your completed purification is lane ____________ This lane would correlate with a ____________ in specific activity.

更多留学生实用工具

希望你的学习变得更简单

You run a gel after a purification experiment. This gel is shown above: The lane that most likely represents your completed purification is lane This lane would correlate with a in specific activity.