the SDS-PAGE gel shown above is the result of an experiment in which a ~55kDa protein is purified from cells, shown in Lane 3. What would you predict lanes 4, 5, and 6 show about the ~55kDa protein? Choose the correct answer from the answers below

Question

BlackTom AI · Accepted Answer

Begin by parsing the scenario: an SDS-PAGE gel is used to analyze a purified ~55 kDa protein from cells, shown in lane 3. The question asks what lanes 4, 5, and 6 would show about the same protein.
Option 1: 'Lane 4, 5, and 6 are unrelated to lane 3.' In SDS-PAGE, if lane 3 contains the purified protein, related lanes (4–6) typically examine the same protein under different conditions or forms. Saying they are unrelated ignores the experimental design and the expected relationship between the lanes, making this choice incorrect.
Option 2: 'The 55kDa protein is made up of smaller subunits shown in lane 4, and independently in lanes 5 and 6.' Under denaturing SDS-PAGE, a protein can dissociate into its subunits. If lane 3 shows the intact ~55 kDa protein, lanes 4–6 could reveal its constituent subunits as separate bands with lower apparent molecular weights. The phrasing 'made up of smaller subunits' reflects the typical interpretation that the single polypeptide is composed of subunits that appear separately when denatured, so this option aligns with the expected interpretation of SDS-PAGE results involving subunit structure.
Option 3: 'Proteins in Lane 4 are phosphorylated while the ones in the other lanes are not.' Phosphorylation can slightly shift migration in some contexts, but SDS-PAGE is not a definitive method to detect phosphorylation status without specific stains or controls. This claim introduces a feature (phosphorylation) that isn’t evidenced by the gel image alone and isn’t the standard interpretation of comparing a purified protein and its potential subunits. Therefore, this option is unlikely as the correct general explanation of lane relationships.
Option 4: 'There is no possible relationship between the proteins shown.' Given that lane 3 contains the same protein and lanes 4–6 are part of the same experimental setup, asserting no relationship disregards basic comparative analysis across lanes and contradicts the common purpose of including multiple lanes in a single gel. This makes the option incorrect.
In summary, the core idea is that SDS-PAGE separates subunits under denaturing conditions, so lanes 4–6 are expected to reveal smaller subunits that compose the ~55 kDa protein, consistent with Option 2. The other options fail because they ignore the experimental link among lanes, misinterpret phosphorylation implications, or claim no relationship where a relationship is implied by the design of the gel.

the SDS-PAGE gel shown above is the result of an experiment in which a ~55kDa protein is purified from cells, shown in Lane 3. What would you predict lanes 4, 5, and 6 show about the ~55kDa protein? Choose the correct answer from the answers below单项选择题

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