Which of the following actions would ensure that a micropipette delivers an accurate and reliable volume of liquid?Note: the term “aspiration/aspirating” in this context refers to the process of bringing a solution into the micropipette.

Question

BlackTom AI · Accepted Answer

A properly matched disposable tip is essential for a good seal and reproducible air column volume, so using a tip sized to the pipette’s volume range is the correct technique.

1) Relevant knowledge points and concepts
- Air-displacement micropipettes measure liquid by displacing an air column; accuracy depends on a tight seal between tip and pipette and a reproducible air column volume.
- Tips are made in size ranges that minimize “dead volume” and ensure a snug, leak-free fit.
- Pipette technique: aspirate with the tip vertical, release the plunger slowly to avoid bubbles, and use the first stop to aspirate; the second stop (blowout) is for dispensing all remaining liquid.

2) Step-by-step reasoning
- If the tip does not match the pipette’s specified range, the fit can be loose (causing leaks) or dead volume can be large, both leading to systematic under- or variable volumes.
- Attaching the correct tip before aspirating ensures the air column is the intended length and the seal is reliable.
- Proper aspiration requires slow, controlled plunger release with the pipette held vertically; these technique points support why the other choices are poor.

3) Calculations
- None required; reasoning is qualitative and based on instrument mechanics.

4) Why the correct answer is correct
- Option b ensures a tight seal and appropriate dead volume, so the displaced air column corresponds to the set volume, producing accurate and reliable delivery.

5) Why other options are incorrect
- a: Snapping the plunger back can draw in air bubbles and cause variable volumes.
- c: Holding the pipette horizontally can allow liquid into the barrel or change the effective column length, causing inaccuracy.
- d: Depressing to the second stop before aspirating will pull excess liquid or damage tips/piston; the second stop is for dispensing, not aspirating.

6) Conclusion
Using a tip that matches the micropipette’s volume range before aspirating (option b) is correct and matches the provided answer.

Which of the following actions would ensure that a micropipette delivers an accurate and reliable volume of liquid?Note: the term “aspiration/aspirating” in this context refers to the process of bringing a solution into the micropipette.Single choice

Similar Questions

使用移液器吸取液体时，活塞应该按压到哪个位置？

用移液器吸取液体时，活塞应该按压到哪个位置？

When loading an pipetter with liquid, to which stop do you depress the plunger?

When dispensing liquid from an autopipette, to which stop do you depress the plunger?

Which of the following actions are recommended when releasing fluid from the pipette tip into a container?

Which of the following actions are recommended when releasing fluid from the pipette tip into a container? 在将液体从移液器尖端释放到容器时，推荐以下哪些操作？

For standard aspiration, press the plunger to the [ Select ] first second third stop. For dispensing, press to the [ Select ] first second third stop to dispense the bulk of the liquid, then continue to the [ Select ] second first third stop to blow out any remaining droplets.

More Practical Tools for Students Powered by AI Study Helper

Homework AI Solver

Stylized AI Paper Writer

Plagiarism Checker Assistant

Citation AI Academic Writing Tool

In-Class Translation Assistant

AI Note Generator

AI Quiz Answers

Past Exam Questions from University Test Bank

Smart Practice Assistant

Adaptive Practice

Making Your Study Simpler