Which of the following actions would ensure that a micropipette delivers an accurate and reliable volume of liquid?Note: the term “aspiration/aspirating” in this context refers to the process of bringing a solution into the micropipette.

Question

BlackTom AI · Accepted Answer

A properly matched disposable tip is essential for a good seal and reproducible air column volume, so using a tip sized to the pipette’s volume range is the correct technique.

1) Relevant knowledge points and concepts
- Air-displacement micropipettes measure liquid by displacing an air column; accuracy depends on a tight seal between tip and pipette and a reproducible air column volume.
- Tips are made in size ranges that minimize “dead volume” and ensure a snug, leak-free fit.
- Pipette technique: aspirate with the tip vertical, release the plunger slowly to avoid bubbles, and use the first stop to aspirate; the second stop (blowout) is for dispensing all remaining liquid.

2) Step-by-step reasoning
- If the tip does not match the pipette’s specified range, the fit can be loose (causing leaks) or dead volume can be large, both leading to systematic under- or variable volumes.
- Attaching the correct tip before aspirating ensures the air column is the intended length and the seal is reliable.
- Proper aspiration requires slow, controlled plunger release with the pipette held vertically; these technique points support why the other choices are poor.

3) Calculations
- None required; reasoning is qualitative and based on instrument mechanics.

4) Why the correct answer is correct
- Option b ensures a tight seal and appropriate dead volume, so the displaced air column corresponds to the set volume, producing accurate and reliable delivery.

5) Why other options are incorrect
- a: Snapping the plunger back can draw in air bubbles and cause variable volumes.
- c: Holding the pipette horizontally can allow liquid into the barrel or change the effective column length, causing inaccuracy.
- d: Depressing to the second stop before aspirating will pull excess liquid or damage tips/piston; the second stop is for dispensing, not aspirating.

6) Conclusion
Using a tip that matches the micropipette’s volume range before aspirating (option b) is correct and matches the provided answer.

Which of the following actions would ensure that a micropipette delivers an accurate and reliable volume of liquid?Note: the term “aspiration/aspirating” in this context refers to the process of bringing a solution into the micropipette.单项选择题

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