Which of the following actions are recommended when releasing fluid from the pipette tip into a container?

Question

BlackTom AI · Accepted Answer

Air-displacement pipettes have two defined plunger positions: the first stop dispenses the set volume, and the second stop is the “blow‑out” position used to expel residual liquid. Also, proper tip orientation (a slight angle and contact with the vessel wall) helps liquid flow off the tip cleanly and reduces splashing or aerosol formation.

1) Relevant knowledge points and concepts
- Plunger stops: first stop = dispense set volume; second stop = blow out residual.  
- Tip orientation: slight angle (~20–45°) against the container wall promotes complete drainage.  
- Tip ejector is for removing the disposable tip after dispensing, not for releasing liquid.  
- Holding pipette vertical can cause splashing, inaccurate delivery, or tip contact with vessel bottom.

2) Step-by-step reasoning process
- After aspirating, position the tip just inside or against the side of the receiving container at a slight angle.  
- Depress the plunger to the first stop to deliver the measured volume.  
- Continue to the second stop to blow out remaining liquid from the tip for complete transfer.  
- Withdraw the tip while maintaining the second stop if necessary, then release the plunger and eject the tip separately.

3) Calculations
- None required for technique; no numeric intermediate steps apply.

4) Why the correct answers are correct
- “Push the top button down to the second stop” (a) is correct because the second stop eliminates residual liquid, ensuring full volume is delivered.  
- “Hold the pipette on a slight angle” (d) is correct because angling the tip against the container wall helps liquid run off and prevents splashing or trapped droplets.

5) Why the other options are incorrect
- (b) Holding completely vertically increases risk of splashing or tip-bottom contact and is not recommended.  
- (c) Pushing only to the first stop may leave residual liquid in the tip, giving inaccurate delivery.  
- (e) The tip release button ejects the disposable tip and should not be used to discharge liquid—using it during dispensing can create aerosols and contamination.

6) Conclusion
The recommended actions are (a) push to the second stop and (d) hold the pipette on a slight angle. This matches the provided correct answer: a and d.

Which of the following actions are recommended when releasing fluid from the pipette tip into a container?多项选择题

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