Phage display technology is primarily used to:噬菌体展示技术主要被用于：

Question

BlackTom AI · Accepted Answer

Phage display links a protein’s DNA (genotype) to the protein displayed on a bacteriophage surface (phenotype), enabling selection of binders from vast libraries. In antibody discovery this is typically done by displaying antibody fragments (e.g., scFv or Fab) on filamentous phage coat proteins, then selecting those phage that bind a target antigen.

1) Relevant knowledge points and concepts
- Phage display library: billions of variant peptides or antibody fragments encoded in phage genomes and presented on the phage surface.  
- Biopanning/selection: iterative binding, washing, elution and amplification steps that enrich phage displaying high-affinity binders.  
- Affinity maturation: repeated selection rounds favor higher-affinity clones; sequences recovered reveal antibody variable regions for further development.  
- Scope: discovery and engineering of binding molecules (peptides, antibody fragments), epitope mapping, and affinity optimization—not bulk production of therapeutic mAbs.

2) Step-by-step reasoning process
- Construct a diverse antibody-fragment library and clone into phage display vector so fragments are fused to a coat protein.  
- Incubate the phage library with immobilized target antigen; phage with no/low binding are removed by washing.  
- Elute bound phage, infect bacteria to amplify them, and repeat these “panning” rounds (typically 3–5) to enrich for high-affinity binders.  
- Sequence enriched clones, express chosen antibody fragments recombinantly, and further characterize/format into full antibodies if needed.

3) Calculations
- Not applicable for this conceptual selection technique.

4) Why the correct answer is correct
- The described workflow explicitly selects antibody fragments that bind a specific antigen with high affinity; phage display directly enables discovery and engineering of these fragments, matching option (c).

5) Why the other options are incorrect
- (a) Measuring Fc receptor binding: Fc interactions concern the antibody Fc region and receptor assays—these are functional/biophysical tests, not the primary purpose of phage display.  
- (b) Large-scale mAb production: manufacturing uses mammalian cell expression (CHO cells), not phage display. Phage display is a discovery/engineering tool, not a production platform.  
- (d) Reducing immunogenicity by Fc humanization: humanization is a sequence-engineering process (grafting or redesign) and subsequent testing; phage display may be used for some engineering steps but is not specifically an Fc-humanization production method.

Conclusion: Phage display is primarily used to select antibody fragments with high affinity toward a specific antigen, so the correct answer is (c), which matches the provided answer.

Phage display technology is primarily used to:噬菌体展示技术主要被用于：单项选择题

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