What is the purpose of a DNA ladder or molecular weight marker in gel electrophoresis?单项选择题

A

To amplify DNA samples

B

To provide a reference for estimating the sizes of DNA fragments

C

To color the gel

D

To improve gel resolution

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类似问题

Question textTo calculate the sizes of your restriction digest fragments, you will need to construct a standard curve of the migration rate of the DNA markers against their log10 size in base pairs. The table below is provided to tabulate the data necessary for making this graph.Calculate the Log10 size of each DNA marker, and input this into the table. Following the instructions present on the LMS, measure the migration for each DNA marker and record this in the table as well.NOTE: Only the middle column (Log10 size of DNA marker) will be graded. Do not enter any units (i.e. bp) in this column and give your answer to 2 decimal points.[table] Size of DNA marker (bp) | Log10 size of DNA marker (bp) | Distance migrated by DNA marker from top of agarose gel (mm) 15000 | Answer 1 Question 8 | Answer 2 Question 8 10000 | Answer 3 Question 8 | Answer 4 Question 8 8000 | Answer 5 Question 8 | Answer 6 Question 8 7000 | Answer 7 Question 8 | Answer 8 Question 8 6000 | Answer 9 Question 8 | Answer 10 Question 8 5000 | Answer 11 Question 8 | Answer 12 Question 8 4000 | Answer 13 Question 8 | Answer 14 Question 8 3000 | Answer 15 Question 8 | Answer 16 Question 8 2000 | Answer 17 Question 8 | Answer 18 Question 8 1500 | Answer 19 Question 8 | Answer 20 Question 8 1000 | Answer 21 Question 8 | Answer 22 Question 8 850 | Answer 23 Question 8 | Answer 24 Question 8 650 | Answer 25 Question 8 | Answer 26 Question 8 500 | Answer 27 Question 8 | Answer 28 Question 8 400 | Answer 29 Question 8 | Answer 30 Question 8 300 | Answer 31 Question 8 | Answer 32 Question 8 200 | Answer 33 Question 8 | Answer 34 Question 8 100 | Answer 35 Question 8 | Answer 36 Question 8 [/table]

Which fluorescent reagent will you use to visualize DNA in an agarose gel by virtue of its ability to intercalate between base pairs of DNA?

Which DNA molecules move furthest from the origin during agarose gel electrophoresis, and why?

Reference the gels shown above: What is the approximate size of the protein found in the liver? What is the main force pushing this protein down the gel (i.e. protein wouldn't move without this) What are the "hurdles" or pores that slow down the protein as it moves through the gel?

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