A student performed a flow cytometry experiment to label mouse spleen cells with fluorochrome conjugated monoclonal antibodies against mouse CD3 and CD4. The labelled cells were run through a flow cytometer and analysed. The result is presented below.

What is this type of figure often called?

Question

A student performed a flow cytometry experiment to label mouse spleen cells with fluorochrome conjugated monoclonal antibodies against mouse CD3 and CD4. The labelled cells were run through a flow cytometer and analysed. The result is presented below.

What is this type of figure often called?

BlackTom AI · Accepted Answer

The question asks: What is this type of figure often called?

Option 1: A hot plot. While some cytometry software can display density or heat-mapped representations labeled as 'hot plots,' the classic two-parameter flow cytometry representation that shows individual events as discrete points is not typically called a hot plot. This choice can be misleading because 'hot plot' more commonly refers to a density heat map, not a standard scatter representation.

Option 2: A dot plot. This is the standard term for a two-parameter scatter plot in flow cytometry where each event (cell) is represented by a dot on a two-dimensional axis (here CD3 vs CD4). The figure displays individual events with color indicating density, but the fundamental representation is a dot plot, which aligns with the described scatter of CD3 and CD4 expression.

Option 3: A histogram. A histogram shows the distribution of a single parameter by bars along one axis. It does not plot two parameters against each other, so it does not match the provided two-parameter scatter layout.

Option 4: A contour plot. A contour plot displays lines of constant density or value on a 2D plane, representing density levels rather than individual events. While the data could be represented as contours, the given image emphasizes individual points, which is characteristic of a dot plot.

Option 5: A density plot. A density plot uses color intensity to represent the density of events in a region, typically without plotting each event as a separate dot. The image shows many dispersed points with color, but the common representation remains a dot plot with density coloring, not a pure density plot.

In summary, the classic, widely accepted name for this two-parameter, event-by-event scatter representation in flow cytometry is a dot plot. The other options describe related but distinct representations and do not fully capture the standard two-parameter scatter display with individual events.

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