This question requires you to use the flow cytometry result below:

In the figure shown above, the group of spleen cells in the lower left quadrant would best be defined as what?

Question

This question requires you to use the flow cytometry result below:

In the figure shown above, the group of spleen cells in the lower left quadrant would best be defined as what?

BlackTom AI · Accepted Answer

To tackle this flow cytometry question, first identify what the axes and quadrants represent. The x-axis is CD4-PE and the y-axis is CD8-APC, so each cell population can be categorized by being CD4 positive or negative and CD8 positive or negative. The lower left quadrant corresponds to cells that are negative for both CD4 and CD8 (CD4– and CD8–).

Option 1: B lymphocytes. B cells are typically not defined by CD4 or CD8 status on standard T-cell panels; they are usually CD19+ or CD20+ when identified. This quadrant analysis specifically uses CD4 and CD8 markers, so labeling this group as B lymphocytes without CD4/CD8 positivity is not appropriate.

Option 2: CD8– CD4+ splenocytes. This population would appear in the lower right quadrant (CD4 positive, CD8 negative) because CD4 is positive and CD8 is negative. Since the question asks about the lower left quadrant (CD4–, CD8–), this option does not match.

Option 3: CD8+ CD4– splenocytes. This group would be in the upper left quadrant (CD4 negative, CD8 positive). The lower left quadrant is negative for both markers, so this is not the correct region.

Option 4: CD8+ CD4+ splenocytes. These cells would plot in the upper right quadrant (positive for both CD4 and CD8). The lower left quadrant corresponds to double negatives, so this option is incorrect for quadrant mapping.

Option 5: CD8– CD4– splenocytes. This is exactly the double-negative population that lies in the lower left quadrant on a CD4 vs CD8 plot. It matches the description of cells negative for both markers.

Option 6: Cytotoxic T lymphocytes. Cytotoxic T cells are typically CD8+ (and often CD3+), which would place them in the CD8+ quadrant, not in the CD4– CD8– double-negative region. Therefore this option does not describe the lower left quadrant.

Option 7: Helper T lymphocytes. Helper T cells are typically CD4+ (CD4 positive, CD8 negative), placing them in the lower right quadrant, not the lower left. Thus this option is not correct for the given quadrant.

Overall, the lower left quadrant corresponds to cells that do not express either CD4 or CD8, i.e., CD8– CD4– splenocytes. The other options either place cells in different quadrants based on their CD4 or CD8 status or misidentify the population entirely.

This question requires you to use the flow cytometry result below: In the figure shown above, the group of spleen cells in the lower left quadrant would best be defined as what?Single choice

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