Which of the following are valid methods for detecting doublets in single-cell RNA-seq experiments? (Select ALL that apply)多项选择题

A

Using hashtag oligonucleotide (HTO) antibody barcodes to label cells from different samples

B

Loading cells from two different species and identifying mixed transcriptomes

C

Reducing the cell loading concentration to lower doublet rate

D

Using genetic barcoding (demuxlet) to identify cells with mixed genotypes from different individuals

E

Increasing the number of PCR amplification cycles

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