To what speed is the centrifuge set when washing the DNA in the spin column?

Question

BlackTom AI · Accepted Answer

10,000 rpm — this is the standard spin speed used during the wash steps of silica spin‑column DNA purification.

Relevant knowledge points and concepts:
- Silica spin columns bind DNA under high‑salt conditions; wash buffers remove salts and contaminants while DNA remains bound to the membrane.
- Centrifugation forces the wash solution through the membrane; the protocol specifies a minimum speed to ensure complete flow‑through and efficient removal of contaminants.
- Typical kit instructions call for a medium‑high microcentrifuge speed (commonly ~10,000 rpm or the equivalent g‑force) for wash steps; much lower speeds leave liquid on the column, and much higher speeds are unnecessary and may risk splashing or exceed equipment limits.

Step‑by‑step reasoning:
1. Identify the purpose of the step: washing DNA bound to the column to remove impurities.
2. Determine the functional requirement: generate sufficient centrifugal force to push wash buffer through the silica membrane in the allotted time.
3. Compare the answer choices to standard protocols: 2,000 rpm and 5,000 rpm are typically too low to reliably clear wash buffers from the column; many kit manuals explicitly recommend higher speeds.
4. 20,000 rpm is higher than commonly recommended for routine wash steps (often reserved for pelleting small particles or for specific applications) and may exceed the safe operating speed for some tabletop microcentrifuges or be unnecessary for effective washing.
5. 10,000 rpm matches the conventional manufacturer instructions and balances effective flow with equipment safety and protocol design.

Why the correct answer is correct:
10,000 rpm provides the recommended centrifugal force to clear wash buffer and salts from the column quickly and reproducibly, matching standard spin‑column DNA purification protocols.

Why other options are incorrect:
- 2,000 rpm: insufficient force; wash buffer may not pass through completely.
- 5,000 rpm: still generally too low for reliable washing.
- 20,000 rpm: excessive/unnecessary for this step and not the typical instruction.

Final confirmation: The correct choice is 10,000 rpm, which matches the provided answer.

To what speed is the centrifuge set when washing the DNA in the spin column?单项选择题

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