From the DNA sequence 5' ATGGCGT 3', what is expected to be its complementary DNA sequence?

Question

BlackTom AI · Accepted Answer

Base-pairing rules: A pairs with T and G pairs with C, and DNA strands are antiparallel (one runs 5'→3' and the complementary runs 3'→5'). Start with the given strand and determine the complementary bases, then present the complementary strand in the standard 5'→3' orientation.

Original strand (given): 5' A T G G C G T 3'

Step 1 — find complementary bases for each position (reading left to right along the given 5'→3'):
- A (pairs with) T
- T → A
- G → C
- G → C
- C → G
- G → C
- T → A

If you write those complements directly under the original bases (keeping the same left-to-right order), you obtain the complementary sequence running 3'→5' (because base-pairing produces an antiparallel strand):
3' T A C C G C A 5'

Step 2 — convert this into the conventional 5'→3' presentation by reversing the order:
Reverse 3' T A C C G C A 5' to get 5' A C G C C A T 3'

Thus the complementary strand, written in 5'→3' orientation, is 5' ACGCCAT 3'.

Why this is correct: the antiparallel nature requires reversing the complemented sequence to present it in 5'→3' order. A common mistake is to report the complement without reversing (for example writing 5' TACCGCA 3'), which would be the sequence complementary bases but in the wrong orientation. Since no other answer options are provided here, the usual incorrect possibilities are either wrong base-pair assignments or failure to reverse the strand orientation.

Final confirmation: the correctly determined complementary DNA sequence is 5' ACGCCAT 3', matching the provided answer.

From the DNA sequence 5' ATGGCGT 3', what is expected to be its complementary DNA sequence?单项选择题

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