Question text WORKSHOP QUESTION Refer to the bacterial growth data above presented in the workshop. Using only the following terms, fill in the blanks (you can copy and paste the terms between commas): acetate, acetate utilisation, arginine, arginine utilisation, glucose, glucose utilisation, glycerol, glycerol utilisation, histidine, histidine utilisation, destruction, no growth, robust growth, partial growth, DNA repair, carbon utilisation, nitrogen utilisation, gain no new information, determine a redundancy in function, observe that together they are essential genes NOTE: not all the above terms are required, some may be used more than once and all terms must be spelled correctly (excluding the commas) to be marked as correct. --- When gene D is deleted we observe no growth when Answer 1 Question 8[input] is the sole source in the media, with partial growth when exposed to only Answer 2 Question 8[input] as a source in the media. When the gene E mutant is exposed to only glycerol as a carbon source we observe Answer 3 Question 8[input] of the cells. The function of gene E appears to be related to Answer 4 Question 8[input]. When observing the phenotypes of the gene B mutant, it appears to be essential for Answer 5 Question 8[input]. From the result of the A&B double mutant across the growth conditions compared to the single mutants, we can Answer 6 Question 8[input]. From the result of the C&D double mutant across the growth conditions compared to the single mutants, we can Answer 7 Question 8[input].多项填空题

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Question textAn E.coli mutant liquid bacterial culture is grown overnight in nutrient broth at 37 degrees.The next day 10 μl of this culture is diluted in 90 μl of saline (dilution 1), then 10 μl of dilution 1 is diluted in 90 μl of saline (dilution 2).All 100 μl of dilution 2 is then spread on a nutrient agar (NA) plate which is incubated at 37 degrees for 24 hours; 20 bacterial colonies are counted on the NA plate.a) What is viable count (VC) of the original, undiluted liquid culture in "cells per ml"?Answer 1 Question 1[input] cells per ml Give a whole number numerical answer. Do not include "cells per ml"(1 mark)1 ml of a second, undiluted culture with a VC of 50,000 cells per ml is concentrated by centrifugation, resuspended in saline and spread on a minimal media (MM) plate which is incubated at 37 degrees for 24 hours; 5 bacterial colonies are counted on the MM plate. The original mutant bacterial strain was not able to grow on minimal media but wild type E.coli can.b) What is the rate of reversion to wild type for this mutant strain in "revertants per 1000 cells"?Answer 2 Question 1[input] revertants per 1000 cells Give a numerical answer up to maximum three decimal places. Do not include "revertants per 1000 cells"(1 mark)In a parallel experiment performed as above, an unknown chemical, X, was added to the same mutant bacterial strain during the growth phase in nutrient broth. This time, 400 bacterial colonies were counted on the MM plate.c) Therefore, chemical X is acting as a(n) Answer 3 Question 1[input] Give a single word answer(1 mark)

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