All calibration standards contain not only ketoprofen (the analyte), but also uracil as an additive. Our solvent system will be slightly acidic (acetonitrile:water 70:30 plus 0.1 % formic acid) and we will use a reverse-phase column. Which of the two compounds will elute first? Ketoprofen (image: https://webbook.nist.gov/) Uracil (image: https://webbook.nist.gov/)单项选择题

题目图片
A

a. Ketoprofen

B

b. Uracil

C

c. Both will elute at the same time

D

d. Neither will elute in the sample time

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All calibration standards contain not only ketoprofen (the analyte), but also uracil as an additive.所有校准标准不仅含有酮洛芬（分析物），还含有尿嘧啶作为添加剂。 Our solvent system will be slightly acidic (acetonitrile:water 70:30 plus 0.1 % formic acid) and we will use a reverse-phase column.我们的溶剂系统将为微酸性（丙腈：水70：30加0.1%甲酸），并使用反相色谱柱。 Which of the two compounds will elute first?两种化合物中哪一种会先被洗脱？ Ketoprofen (image: https://webbook.nist.gov/) 酮普洛芬（图片来源：https://webbook.nist.gov/） Uracil (image: https://webbook.nist.gov/) 尿酸（图片：https://webbook.nist.gov/）

所有校准标准不仅含有酮洛芬（分析物），还含有尿嘧啶作为添加剂。 我们的溶剂系统将为微酸性（丙腈：水70：30加0.1%甲酸），并使用反相色谱柱。 两种化合物中哪一种会先被洗脱？ 酮普洛芬（图片：https://webbook.nist.gov/） 尿酸（图片：https://webbook.nist.gov/）

The implementation and loading phase of the Database Life Cycle (DBLC) involves _____.

Question textComplete the following protocol table for setting up your standard curve for protein. Remember, each of your standards, except the reagent blank, will be prepared in duplicate in the lab (i..e, you will make up each of these standards twice), giving you 11 standards. When filling in your answers, be careful with your formatting and note the following requirements: for all entries of concentrations, volumes and amounts in this table, include two decimal places (e.g. '1.5') for any decimal numbers less than one, make sure you precede the decimal point with the number zero (i.e. type in '0.2'; not '.2' without the zero) [table] Standard | Concentration of BSA standard | Volume of 20 mg/mL BSA stock solution required (mL) | Volume of 0.05 M NaOH diluent required (mL) | Amount of BSA present (mg) | Volume (mL) 1(reagent blank) | 0 | Answer 1 Question 6 | Answer 2 Question 6 | Answer 3 Question 6 | 0.8 2 | 4 | Answer 4 Question 6 | Answer 5 Question 6 | Answer 6 Question 6 | 0.8 3 | 8 | Answer 7 Question 6 | Answer 8 Question 6 | Answer 9 Question 6 | 0.8 4 | 12 | Answer 10 Question 6 | Answer 11 Question 6 | Answer 12 Question 6 | 0.8 5 | 16 | Answer 13 Question 6 | Answer 14 Question 6 | Answer 15 Question 6 | 0.8 6 | 20 | Answer 16 Question 6 | Answer 17 Question 6 | Answer 18 Question 6 | 0.8 [/table]

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