Iron is an essential nutrient that is acquired by organisms from the environment. When intracellular levels of iron are relatively high, living cells synthesize an iron-storage protein called ferritin. The induction of ferritin synthesis by iron was investigated in rats. Figure 1 shows the results of an experiment in which cellular levels of ferritin protein were measured in the presence or absence of iron and actinomycin D, a drug that inhibits transcription. Figure 2 shows the results of an experiment in which cellular levels of ferritin protein were measured in the presence or absence of iron and cycloheximide, a drug that inhibits translation. Which of the following biotechnology approaches could be used to identify ferritin mRNA in a sample of total cellular RNA?单项选择题

题目图片
A

RNA samples could be converted to protein and subsequently cut with a restriction endonuclease that recognizes DNA sequences.

B

RNA samples could be separated by size using agarose gel electrophoresis and incubated with labeled single-stranded DNA molecules that are complementary to the ferritin mRNA.

C

RNA samples could be examined under a high-power microscope to visually identify the ferritin mRNA.

D

RNA samples could be directly cloned into a DNA plasmid, grown in bacteria, and tested for the ability to bind iron.

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