In a flow-through AEX step, which statement is NOT correct?

Question

BlackTom AI · Accepted Answer

Question restatement: In a flow-through AEX step, which statement is NOT correct?

Option 1: 'Product is primarily collected in the column effluent'
- In flow-through anion exchange, the product typically passes through to the effluent while impurities bind to the resin. This statement is largely correct as the product is collected in the effluent, so it describes the general outcome of a flow-through AEX step.

Option 2: 'The step is often used as a polishing step'
- Flow-through AEX steps are commonly used for polishing, removing impurities at a later stage without binding the product to the resin, which aligns with common practice. This statement is correct.

Option 3: 'Many impurities are retained on the resin'
- This is a defining feature of flow-through mode: impurities are captured by the resin while the target product passes through. This statement is correct.

Option 4: 'Product is eluted using a salt or pH change'
- In flow-through AEX, the product does not rely on a salt or pH shift to elute from the resin; rather, the product is collected in the effluent while impurities are retained. Elution of the product by salt or pH change is characteristic of bind-and-elute modes, not flow-through. This statement is incorrect and therefore NOT correct about flow-through AEX.

Putting these together, options 1–3 accurately describe flow-through AEX characteristics, while option 4 describes a bind-elute mechanism rather than flow-through. The incorrect statement is that the product is eluted using a salt or pH change, which does not define flow-through behavior in AEX.

In a flow-through AEX step, which statement is NOT correct?Single choice

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