Which of the following is the DNA template for this sequence?

5’ AGUAAUCUG 3’

Question

Which of the following is the DNA template for this sequence?

5’ AGUAAUCUG 3’

BlackTom AI · Accepted Answer

RNA is synthesized 5'→3' complementary to the DNA template strand, which therefore runs 3'→5'. To find the DNA template for 5' AGUAAUCUG 3', take each RNA base and replace it with its complementary DNA base (A→T, G→C, U→A, C→G), keeping the antiparallel orientation (template written 3'→5').

Step-by-step base pairing (RNA 5' → DNA template 3'):
RNA:  5'  A  G  U  A  A  U  C  U  G  3'
DNA : 3'  T  C  A  T  T  A  G  A  C  5'

Mapped one-by-one:
- RNA A (5' end) pairs with DNA T → first base of template (3') is T.
- RNA G pairs with DNA C → next base C.
- RNA U pairs with DNA A → next base A.
- RNA A → DNA T.
- RNA A → DNA T.
- RNA U → DNA A.
- RNA C → DNA G.
- RNA U → DNA A.
- RNA G (3' end of RNA) → DNA C (5' end of template).

Thus the template strand is 3' TCATTAGAC 5'.

Why other choices are incorrect:
- "3’ UCAUUAGAC 5’" includes U (uracil), which does not occur in DNA; DNA uses T.
- "5’ AGTAATCTG 3’" is the DNA coding (non-template) strand sequence (it matches the RNA except T instead of U) and is written 5'→3' rather than the required 3'→5' template orientation.
- "3’ AGTAATCTG 5’" has the coding-sequence bases but in the 3'→5' orientation; it is not the template (it is the complement of the RNA).

Therefore the correct DNA template is 3’ TCATTAGAC 5’, matching the provided answer.

Which of the following is the DNA template for this sequence? 5’ AGUAAUCUG 3’ Single choice

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